Minimum Replication and Decision Rules for Presence/Absence Calls from Soil eDNA Metabarcoding
Registry identifier: FOAR-CON2X-KHW78203P
Version: 1.0
Status: Accepted for registration
Publication date: 21 September 2015, 12:00 UTC
Document type: Procedure or protocol
Subject area: Biology and biodiversity
Licence: Creative Commons Attribution 4.0
Scope
Version 1.0 replaces the fixed triplicate/single-positive decision in 0.8 with an error-aware protocol. It governs how a soil-eDNA biodiversity project chooses PCR replication, preserves each replicate, and reports present, absent, indeterminate, or invalid calls. It excludes abundance inference, field sampling, primer design, clinical material and taxonomic revision.
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Versions and documentary supplements
- Version 1.0, 21 September 2015, 12:00 UTC (
FIAS-SUB-2015-BIO-02) MARC